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PROCEDURE AND THE ORGANIZATION OF RESEARCHES

Researches are spent to «Laboratories of medical and biologic problems of the person» Tver state university with participation of 85 examinees - of women of three age-grades agree the CART (1965: 25-44) years - young, 45-59 years - average, 60-75 years - elderly (tab.

1). Experimental bunches - teachers of high school, control - not engaged in teaching work.

Table 1

Allocation of examinees on age-grades

Age-grades Quantity of examinees
25-44 years 45-59 years 60-75 years
The experimental 17 16 15
The control 12 13 12

1st series of researches included: 1) studying CHSS and system the HELL, the account veso-rostovyh indicators, according to B.A.Nikitjuka's recommendations (1998);

2) record of duration KI with subsequent analysis RS. Researches included also calculation of an index of mass of a body on Ketle (IMT, c.u.). Measurement system the HELL was spent with use of automatic measuring instrument UA-777 AC (AnD, Japan), registering in mm hg systolic (GARDEN) both diastolic (DAD) and settlement pulse pressure (PD) is triple with a finding of average value. In 2nd series registration and analysis REG for the purpose of an assessment of a state of veins in polusharnoj and stvolovoj brain ranges are carried out. The biochemical analysis of blood of examinees is spent to 3rd series with accent on lipidnyj a profile comparatively with SSS.

Planning and carrying out of researches are co-ordinated with committee on bioethics TvGU in respect of informing of examinees on participation in researches.

Research of a rhythm of warm reductions. Registration RS was spent in first half of day after 5-minute acclimatisation in a prone position at free breath in the conditions of relative rest. Record kardiointervalov was carried out by means of the computer program «Pulse» with pulsotahometra O-84 and the reformative device, and also is selective on reografe - polyanalyzer "РЕАН-ПОЛИ-6/12" with the built in program of analysis RS. Preliminary analysis KI is spent taking into account recommendations G.D. Clifford et al. (2006), N.I.Jabluchansky and A.V.Martynenko (2010).

Processing of records included: 1) calculation of frequency of warm reductions (CHSS, ud/mines), average duration of cycles (X, c), a coefficient of variation (Cv, %); 2) variation pulsometriju on R.M.Baevskomu with variation scope (AH,), fashion indicators (Mo,) and its voltages (AMo, %), strain indexes (IN, c.u.) and activity reguljatornyh systems (PARS, points);

3) spectral analysis with calculation of the general vigour of a spectrum (OCH), absolute (мс2) and relative (%) of vigours high-frequency (VCH: 0,4-0,15 Hz), low-frequency (LF: 0,15-0,04 Hz) and "very low-frequency (ONCH: 0,04­0,003 Hz) ranges, indexes simpatovagusnyh interrelations (NCH/VCH, c.u.) and activity of the subcortical centres (ONCH/OCH, %), normirovannyh values Vch - and LF-ranges (LF, n.e.; VCH, n.e.). For analysis of 5-minute records RS and their physiological interpretation R.M.Baevskogo and G.G.Ivanov's recommendations (2003) taking into account R.M.Baevskogo's data, etc.

(1984) are used.

Research of a cerebral hemodynamics by a method tetrapoljarnoj REG was spent after 5-minute acclimatisation of examinees in position sitting at fronto-mastoidalnyh (FM) and oktsipito-mastoidalnyh (OM) localisations of electrodes, on the right and at the left. Record was spent on a diagnostic complex "Valenta" at speed of 50 mm/with and on «РЕАН-ПОЛИ-6/12», presented in methodical section. Analysis REG included calculation of peak and time indicators, and also indexes (M.A.Ronkin, L.B.Ivanov, 1997): 1) reografichesky
Index (RI, c.u.); 2) asymmetry quotient (KA, %); 3) speed fast (Ub, the Ohm/c) and slow krovenapolnenija (Mind, the Ohm/c); 5) venous outflow (IN, %).

Blood biochemical analysis. It is spent on the basis of kliniko-diagnostic laboratory of the medical centre of Open Company "Belief" (Tver) with participation of 24 examinees of both sexes (13 - comparison bunch, 11 - control) in a range of 40-70 years. Blood took from an elbow vein on an empty stomach approximately through 12 ch. After food intake, between 7 and 9 o'clock in the morning in standard conditions. Indicators lipidnogo a profile are investigated: the general cholesterol (HS, mmol/l), cholesterol lipoproteidov high density (HS LPVP, mmol/l), cholesterol lipoproteidov low density (HS LPNP, mmol/l), triglitseridy (TG, mmol/l). Components lipidogrammy defined enzimaticheskim a method on multichannel analyzer HITACHI. Index calculation aterogennosti news agency (c.u.), the maintenance of the general lipids is carried out (OL, g/l) defined kolorimetricheski on staining reaction with sulfovanilovym a reactant (V.S.Kamyshnikov, 2004).

Statistical analysis of experimental data included mathematical expectation calculation (X±m), dispersions (D) and a standard deflection (σ). Reliability of intergroup distinctions of studied indicators it was defined parametrical t-criterion Stjudenta for independent vyborok and Fisher's F-criterion (p ≤∙ 0, C ^ p≤0,01). Correlation and regressionnyj analysis of data is spent in pair and longitudinalnom variants for the purpose of definition of forms of dependence between signs and their variations with the forecast for probability of results of forthcoming researches. Computer data processing was carried out by means of a software package of statistical analysis STATISTICA 6.0 (StatSoft) and appendices MS Excel 2010 (MS Office).

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Scientific source Ignatyev Daniel Igorevich. the AGE CHARACTERISTIC of the HEART RHYTHM And the STATE of CEREBRAL POTS Taking into account RESULTS of ANALYSIS of BLOOD At TEACHERS of HIGH SCHOOL. The dissertation AUTHOR'S ABSTRACT on competition of a scientific degree of a Cand.Biol.Sci. Tver - 2015. 2015

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