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Multidimensional analysis of results of Ik-spectroscopy

On the basis of dispersion matrixes multidimensional analysis has been carried out and criterion Bartletta is calculated, which has allowed in the presence of the standard (bidistillirovannoj waters) to define degree of distinction of dispersions of analyzed systems on the basis of mathematical expression:

W=b * (-2lnv) where parametres b and-2ln ν are defined under formulas: b=1 - (Z2J=1 (nJ-1))-1/-Z2J=1 (nJ-1)) *3,13;

-2lnv = - (Z2j=1 (nj-1)) *ln|S |-Z2j=1 (nj-1) *ln|Sj |, where nj - number of lines in the table of data, Sj - incorporated dispersion matrix. The size of multidimensional criterion Bartletta is compared with χ2 α, v (the Hi-square allocation) at a=0,05; v = n1 + n2-2. The given indicator characterises a state of system from positions of its dynamism.

The complete criterion was applied to the account of character of changes of absolute indicators propuskanija investigated modelling solutions - distance Mahalanobisa which paid off at comparison of investigated object with the standard. In this case analyzed average values of indicators propuskanija infra-red radiance of two multidimensional vyborok modelling systems under the formula: Do, e = (Mo - M3) *S3 ^ 1 * (Mo - Me), where Moи Me - vectors of averages vyborok "sample" and "standard" accordingly, S√1 - anatropic dispersion matrix of "standard", - a transposing sign.

Thus, criteria Mahalanobisa and Bartletta have allowed to estimate static and dynamic properties of a state. Scatter diagramme use in co-ordinates of these indicators for graphic and analytical interpretation of the received results has allowed to define
On the basis of distance analysis degree of affinity of modelling systems to each other and to estimate effects of influence of material on changes of a water component.

Findings of investigation and their discussion. Ik-spectroscopy of biological system - whey of blood of teenagers

Ik-spectroscopy of whey of blood of teenagers healthy and with a delay of mental development

In the Ik-spectrum of whey of blood of teenagers with a delay of mental development concerning the healthy change of width of strips and height of peaks of all basic characteristic bunches (fig. 1) is established.

So, in a range of length of a wave of 3462 sm-1 concerning symmetric valency fluctuations of bunches-OH,-NH, the peak height that speaks about the smaller maintenance of materials with the given functional bunches (peptides, lipids) is considerably lowered. Absorption in the field of 3207 sm-1, displaced to 3190 sm-1 and less intensive, can be attributed to symmetric valency fluctuations of connected bunches-OH,-NH.

For valency fluctuations-SN nonsaturated fat acids and the aethers of cholesterol defined in the field of a strip of 3020-3000 sm-1, reduction of intensity and shift aside longer waves (3013­3030 sm-1) is noted. In the Ik-spectrum of whey of blood of children with ZPR the strips concerning to dissymetric (2990-2950 sm-1) and to symmetric fluctuations metilnoj bunches of aethers of cholesterol, triglitseridov, glitserola (2880-2860 sm-1) are not found.

Change of width of a strip of dissymetric valency fluctuations of bunch-SN2 fat acids and phosphotides about 2950-2880 sm-1 to 2957-2870 sm-1 is established.

It is defined insignificant ushirenie a range (with falling of intensity of absorption), characterising the symmetric valency
Fluctuations-SN3 bunches of fat acids and phosphotides, from 2870-2830 sm-1 to 2870-2825 sm-1.

Fig. 1. The Ik-spectrum of whey of blood of teenagers healthy () and with a delay of mental development ()

Difference in intensity of peaks of deformation fluctuations-Ch3i-CH2 (2996-2819 sm-1) specifies in various quantity of fat acids, phosphotides and triglitseridov, i.e. on change of an interrelation lipids/squirrels in whey of a blood plasma of sick children. Substantial growth of the area of a range of valency fluctuations of bunch-S=O, defined in lipids, aethers of cholesterol and triglitseridah (1739-1732 sm - 1) is marked. In a range of 1200-1000 sm-1 where the cores are fluctuations of bunches-about - Р=О phosphotides, fosforilirovannyh proteins, a strip of absorption for whey of a blood plasma of bunch № 1 below control, that speaks about the smaller maintenance of the above-named materials.

In the field of 3600-3450 sm-1 peaks which can be carried to valency fluctuations-OH bunches that speaks about water presence in structure gidratnyh covers of biologically awake materials of blood are found. Range
Deformation fluctuations of moleculas of water of whey of plasma (1645 sm-1) investigated bunch in comparison with control it is authentic more low.

Ik-spectrum of whey of blood of teenagers with ZPR in the course of treatment

It is revealed, that preparation application "Korteksin" leads to fall of values propuskanija in the field of an Ik-spectrum characterising strips of absorption of bunches:-S=O communications (phosphotides), and also communications of bunches-R-O-S and-R, inherent fosfatidilinozitolu, fosfatidiletanolaminu and fosfatidilserinu (1750-1610 sm-1 and 1067-930 sm-1 accordingly), that testifies to activation of a metabolism of phosphotides and augmentation of concentration of the basic representatives of the given class of bonds in whey. It confirms interrelation of metabolic processes of amino acids and lipids [with Luneva E.V., 2007; Kilchevsky M. A, 1976].

For an assessment of influence of a preparation "Korteksin" on a water component of whey of blood have calculated the area of strips of absorption of the Ik-spectrum, characteristic for water (valency, deformation and libratsionnye fluctuations). The received results are presented to tab. 1.

Table 1

Size of strips of absorption of the Ik-spectrum of a water basis of whey of blood at medical product influence "Korteksin" (1М) (r glitsin ≈ alanin> lizin [Bejlina D.S., 2003]. It is Thus established: in concentration the investigated amino acids influence of 10-4 M comparable on force on a water basis while in concentrations 10-3, 10-13 M character of this influence is distinguished.

Ik-spectroscopy of water modelling system of a preparation "Korteksin"

Fure the spectroscopy 1М solution Korteksina has allowed to establish presence of all functional bunches, the bonds entering into its structure. Minor alteration of their position concerning help data
It is bound to their interaction with a water basis of solution. In a spectrum communications of the new materials formed as a result of interaction of bonds, amounting a preparation are not found.

Analysis of the solutions received by delution of initial solution from 101 to 1016 times by means of agrarian and industrial complex "ИКАР", has shown, that the greatest size of dispersions of indicators propuskanija is defined for concentration of 10-2 M in a range of 3085-2832 sm-1, and the least - at delution 1010 in range Ik - a spectrum of 1430-1210 sm-1.

In comparison with solutions of separate amino acids complete criterion Mahalanobisa for preparation delutions "Korteksin" has lower values (fig. 5), that it is possible to explain its stabilising influence on a water component.

Fig. 5. Change of distance Mahalanobisa of water solution of a preparation "Korteksin" (at delution 1М solution in 1016раз) and its approximation

On the basis of the infra-red spectroscopy of the information received by a method about indicators propuskanija and their dispersions the scatter diagramme in complete criteria for modelling solutions of amino acids and a preparation "Korteksin" (fig. 6) has been constructed.

In drawing it is visible, that investigated materials form isolated for each of them ranges.

It is revealed, that neutral and sour amino acids settle down on the diagramme along axis Mahalanobisa while Liz forms the range located mainly along axis Bartletta that can characterise ability of material to influence dynamic properties of solution.

Fig. 6. The Scatter diagramme of modelling solutions of amino acids and a preparation "Korteksin"

Thus the preparation "Korteksin" at which there are all investigated amino acids, makes specific impact on a water component, compactly settling down on the diagramme. The given fact, probably, is bound to a hydrophobic interaction of all moleculas of a preparation, high local concentration is thus created at low general concentration. In this case the concentration necessary for exhibiting of effect of medicinal material, will be reached at an interrelation: the above propensity to formation of waterproof communications between components, the less concentration of material in solution.

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Scientific source Butavin Nikita Jurevich. INFLUENCE ENDO - And EKZOGENNYH FACTORS ON the INFRA-RED SPECTRUM of WHEY of BLOOD of TEENAGERS. The dissertation author's abstract on competition of a scientific degree of a Cand.Biol.Sci. Tver - 2014. 2014

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