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2.2 Methods and conditions of carrying out of researches.

Air-dry seeds arabidopsisa specified above saltants

Were sowed in pikirovochnye boxes, the dimension of boxes 15 on 30 the Structure of bedrock and care of plants see are described in one of our works [43].

At a stage of cotyledonous leaves at gibridologicheskom analysis weeding was not spent. Plants were crossed under pauchpym P.D.Usmanov's management. In work the binocular magnifier a/objective of augmentation 2, an eyepiece 15/and thin tweezers was used. At not revealed knops of a saltant 90 left pylniki in 2-3 days when the flower revealed, blossom dust was put on a shaggy pistil under a magnifier hlorofillnyh saltants. These tsvetki were marked, the crop from them collected separately. The second generation of plants the account of the disjoined classes was sowed, effected. Among a class green bulavovidnyh plants have been found

Plants with fastsiirovannym a caulis. From each plant the individual collecting of seeds has been effected. The individual collecting of seeds was effected also from plants fenotipicheski similar to a saltant 90, but with not fastsiirovannym a caulis, and also with yellow and wildings.

Methods of phenological supervision. Phenological supervision were spent each 3 days to a phase butonizatsii, and then each 5 days before the fructification terminal. For supervision for rostovymi processes and the account of quantitative characters 100 modelling plants of each line arabidopsisa undertook. Following parametres of plants were defined: quantity of leaves in the socket, diameter of the socket, number of caulises and branches, caulis height, quantity of pods, quantity of seeds in a pod, width fastsiirovannyh caulises. In the course of fructification efficiency of various strains fastsiirovannyh plants has been defined. Data are statistically treated according to the accepted procedure [51].

Methods of anatomical researches.

Anatomical sections of caulises arabidopsisa became manually by means of the sharp razor. The medium was applied to bracing of anatomical sections JAkovleva (60 ml. The distilled water, 36 ml. 96 % of alcohol, 4 ml. Ice acetic acid, 6 ml. Formalin). To a staining of preparations it was applied metilenovaja a blue [48]. In work microscope МБИ-5 and drawing device RA-4 was applied. Anatomical researches of sections were spent at Institute of phytology AN RT under the guidance of Cand.Biol.Sci. E.P.Zhogolevoj

Methods of quantitative definition of a chlorophyll.

The procedure of quantitative definition of a chlorophyll the offered A.S.evening and So-called Godnevym [8, 16, 49] was used. We applied spectrophotometers Spekol To definition of absorbency of solution of a chlorophyll (effecting Sweden) and M Council of Federation-26 (LOMO). Definition of the quantitative maintenance of a chlorophyll was spent at Institute of phytophysiology and genetics under the guidance of the doctor of the biological
M.B.Nijazmuhamedovoj's sciences, and also in laboratory of organic chemistry TGNU (managing laboratory SH.H. Halikova),

In the dissertation photos of the plants which have been grown up in of Dushanbe are resulted. Photos are made by me, the part of photos is executed by the academician P.D.Usmanovym.

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Scientific source Nikolaev Julia Evgenevna. Physiological and genetic aspects of the phenomenon of fasciation on the example of a model object Arabidopsis thaliana (L.) Heynh. Thesis for the degree of candidate of biological sciences. DUSHANBE - 2006. 2006

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