materials and methods of experimental researches

Experimental researches have been executed on 20 rabbits - males of breed a chinchilla in mass of a body of 2,0-2,5 kg at the age of 4-5 months of Research spent according to Resolution ARVO «On use of animals in experimental researches», rules Good Clinical Practice (GCP) [144, 272].

Chronic OOZES it is reproduced by unitary introduction in an aural vein of normal horse blood serum (manufacture the Bioprize, Russia), warmed up to 37⁰С, in number of 12-15 ml on 1 kg of mass of a body. With a view of a deallergization for the prevention of an allergic shock 24 hours prior to Serum introduction spent preimmunizatsiju (entered 1 ml/kg of same Serum) [171, 172] (fig. 2, fig. 3).

Clinical researches of eyes of rabbits included: biomicroscopy, an ophthalmoscopy and an optical coherent tomography (OST) on the scanner «3 D OCT-1000 MARK II» firms "Topcon" (Japan) (fig. 4). The thickness of a layer of nervous fibers, a pigmental epithelium and horoidei was estimated arhitektonika retina layers.

Photoregistration of an eyeground of animals was made by means of pediatric retinal chamber RetCam II («Clarity Medical Systems», the USA) (fig. 5).

Fig. 2. Normal horse blood serum ("Bioprize", Russia).

Fig. 3. A technique of introduction of normal horse Serum in an aural vein of a rabbit.

Fig. 4. A three-dimensional optical coherent tomograph «3D OCT-1000 MARK II» firms Topcon (Japan).

Fig. 5. Pediatric retinal chamber RetCam II (Clarity Medical Systems, the USA).

Through 1 mes rabbits with modelirovannym chronic OOZES were exposed to influence INEMP, generated apparatus INFITA, on area of a head of animals, within 10 days, distantsionno, through an ultrasonic head located on distance of 20-30 sm from eyes, and intensity of a field in a zone of influence 1-2 мВ/см². Frequency of following of impulses daily varied as 40 - 60 - 80 - 60 - 40 - 60 - 80 - 60 - 40 - 60 Hz (a Fig. 6). Parametres INEMP, generated by apparatus INFITA, were identical to parametres of the physical factor generated by the apparatus INFITA TH.

Fig. 6. A technique of influence INEMP on experimental animals.

For studying of the immune status and an estimation of influence INEMP on system immunovospalitelnyj process at animals of skilled and control group immunologic researches on the basis of FNKTS Children's haematology, oncology and immunology of Dmitry Rogatchyov together with d.m.n are carried out., professor S.A.Rumjantsevym.

Two groups of animals have been generated: healthy rabbits who did not subject to influence of the impulsive low-frequency
Electromagnetic field (n=10), rabbits with a chronic inflammatory pathology (n=10), surveyed before influence INEMP and after the termination 10 day courses of influence INEMP. As object of research blood from an aural vein of rabbits [176] served.

Calculation of blood cells made with use of the automatic analyzer of blood cells Pentra 60 C + Horiba AVH (France). On flowing cytometer FACSCalibur «Becton Dickinson», (USA) in reaction of a direct immunofluorescence taped the lymphocytes carrying membranous markers of this or that subpopulation of immunocompetent cells. By means of an enzyme immunoassay spent quantitative definition in blood serum of cytokines. [103, 176].

Morphological researches of tissues of a kidney of animals of skilled group carried out on chair of histology of medical faculty and chair of morphology of medicobiological faculty GBOU VPO RNIMU of N.I.Pirogova of Ministry of Health of Russia together with the senior lecturer, k.b.n. A.A.Drevalem and the senior lecturer, k.b.n. G.V.Stavitskoj.

Researches of kidneys of animals spent in 45 days after modelling chronic OOZES. Animals deduced from experiment by a narcosis overdosage. Applied a two-componental narcosis: for a premedication entered 2 % a solution ksilozina a hydrochloride (rometar). In 10-16 minutes intravenously in a regional aural vein entered zoletil-50 in a dose more than 6,6 mg/kg of mass of a body [15].

Material for morphological researches were the kidneys of animals received after their preparing. Tissue slices fixed in 2,5 % a solution glutaraldegida on 0,2 M kakodilatnom the buffer, then carried out a lavage in the distilled water. Further spent dehydration in solutions of ascending concentration of acetone and dried up. Semithin sections painted a hematoxylin and eosine.

Research of tissue specimens spent a method of light microscopy, and photoregistration was carried out on the digital chamber in the complete set of apparatus-programnogo complex automatic morfodensitometrii "Diamorfobektiv" ("DiaMorf", Russia).

2.4.