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immunologic methods of research of blood serum

Immunologic researches are executed in the Samara diagnostic centre, in department of laboratory diagnostics.

Immunologic methods defined percentage of subpopulations of lymphocytes (CD4 +, CD16 +) by means of labelled FITTS and fikoeritrinom MKAT series LT ("Sorbent", Moscow).

The immunofluorescence of cells was estimated on laser flowing tsitofljuorimetre “Calibur” (“BD” USA) in regimen CellQuest Pro. Carried out the clinical analysis of blood for definition of absolute quantity of leucocytes (WBC) by means of hematological analyzer Medonic (Sweden).

Absolute maintenance CD4 + - CD16 + - cells (abs h 109 cells of/ml) defined under the formula:

abs lymphocytes h % - noe the maintenance of subpopulation Abs.subpopuljatsii =

100

Estimation of nonspecific and specific functional activity of cells of immune system spent by definition of level of production IFN-y after an incubation integral geparinizirovannoj peripheric blood with FGA and DST, accordingly, within 12 hours at 37С. Level IFN-y defined in supernatante with use IFTS (NPO "Vector-best", Novosibirsk).

2.6.

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Scientific source Ahmerova Tatyana Efimovna. IMMUNODIAGNOSIS OF DIFFERENT MANIFESTATIONS OF TUBERCULOSIS INFECTION IN CHILDREN. Thesis for the degree of candidate of medical sciences. SAMARA - 2014. 2014

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  1. THE TABLE OF CONTENTS
  2. INTRODUCTION
  3. immunologic methods of research of blood serum
  4. THE LITERATURE LIST
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  6. THE LITERATURE LIST
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