Definition of the maintenance of a pyruvate.
Definition of the maintenance of a pyruvate spent as follows: 0,5 g
the blood frozen in liquid nitrogen triturated and ekstragirovali 5мл 5 % THU within 30 minutes, then an extract tsentrifugirovali, to 1 ml supernatanta added 0,5 ml of 0,1 % of a solution 2,4-dinitrofenolgidrazina and in 20 minutes - 5 ml 0, 4 N NaOH.
A solution spektrofotometrirovali at a wavelength of 536 nanometers. Calculation spent under the formula: With = E h 0,59, mmol/l.2.1.2.18.
Scientific source
INGA MAMUCHISHVILI. INTERRELATION STRESS-INDUCED of DISTURBANCES In nejrogumoralnoj to SYSTEM And SYSTEM of POWER SUPPLY At TEENAGERS. The DISSERTATION on competition of a scientific degree of the doctor of medical sciences. Tbilisi - 2006. 2006
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