Definition OVER-N-oksidazy
the Method is based on following transformation:
NADF^JA + 2Î 2 NADFJA-oksidaza __________ NADF + + 2Î - 2 + N +
Took 1 ml resuspenzirovannyh the lymphocytes, containing 50 mg/ml of fiber.
As medium of measurement at research of activity NaDjA-oksidazy used 0,03 mm the phosphatic buffer with 5×0 ’ 4 M EDTA, rja 7,4. Reaction initiated addition OVER TH in final concentration 3 mkmol or cytochrome With - 1,5 mg separately or in common [Almatov K.T., Rakhimov M. M, 1978].Activity OVER-N-OKSIREDUKTAZY raschityvali on rate of oxidation of substrate counted (a decrease OVER TH or cytochrome in nmol/mg of fiber of mines [Kogan A.H. and co-workers., 1999]
2.1.2.8.
Scientific source
INGA MAMUCHISHVILI. INTERRELATION STRESS-INDUCED of DISTURBANCES In nejrogumoralnoj to SYSTEM And SYSTEM of POWER SUPPLY At TEENAGERS. The DISSERTATION on competition of a scientific degree of the doctor of medical sciences. Tbilisi - 2006. 2006
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