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Chapter 5 of FEATURE of the INTESTINAL MICROFLORA AT FUNCTIONAL DISORDERS of the GASTROENTERIC TRACT At CHILDREN of EARLY AGE

For an estimation of a condition of a microflora of an intestine at children of early age with functional disorders of a gastroenteric tract (FRZHKT) we had been carried out following researches: feces crops on a dysbacteriosis, quantitative PTSR a feces and level definition kalprotektina in a feces for an estimation of degree of an inflammation in an intestine.

Results of crops of a feces on a dysbacteriosis

the Analysis of results of crops of a feces on a dysbacteriosis has allowed to tap authentic differences: the quantity bifidobaktery and Staphylococcus aureus in the basic group (OG) was authentically above, than in control group (KG). The quantity laktobaktery, Citrobacter freundii, Klebsiella pneumoniae and Klebsiella oxytoca in OG had no authentic differences in comparison with KG. Results of crops of a feces on a dysbacteriosis in OG and KG are presented in table 26.

Table 26. Results of crops of a feces on a dysbacteriosis in OG and KG

Microorganisms OG

(n=62)

KG

(n=40)

t p
Bifidobakterii 10,75±0,09 10,20±0,29 2,11 =0,03
Laktobakterii 6,77±0,08 6,55±0,15 1,37 =0,17
Citrobacter freundii 4,50±0,15 4,00±0,04 2,63 =0,10
Klebsiella oxytoca 4,77±0,19 4,70±0,24 0,23 =0,81
Klebsiella pneumoniae 5,38±0.21 4,82±0,20 1,80 =0,07
Staphylococcus aureus 3,41±0,27 2,15±0,42 2,62 =0,01

In a drawing 27 the qualitative and quantitative structure of an intestinal microflora according to feces crops on a dysbacteriosis in OG and KG is shown.


WHICH/G

12,00 10,75

10,00

8,00

6,00

4,00

2,00

0,00

10,20

6,77

6,55

4,50

4,00

4,77

5,38

4,70 4,82

3,41

2,15

OG KG

the Drawing 27. Qualitative and quantitative structure of an intestinal microflora according to feces crops on a dysbacteriosis in OG and KG

We have analysed structure of intestinal microflora OG depending on presence LN (FR with LN and FR without LN). According to feces crops on a dysbacteriosis it is taped, that the quantity laktobaktery and Staphylococcus aureus was authentically above at FR with LN, than without LN. The quantity bifidobaktery, Citrobacter freundii, Klebsiella pneumoniać and Klebsiella oxytoca had no authentic differences. Results of crops of a feces on a dysbacteriosis depending on presence LN are presented in table 27.

Table 27. Results of crops of a feces on a dysbacteriosis depending on presence LN

Microorganisms FR with LN (n=23) FR without LN (n=39) t p
Bifidobakterii 11,00±0,23 10,61±0,15 1,48 =0,14
Laktobakterii 7,00±0,09 6,64±0,12 2,10 =0,04
Citrobacter freundii 4,69±0,30 4,38±0,17 0,95 =0,34
Klebsiella oxytoca 5,26±0,40 4,48±0,19 1,91 =0,06
Klebsiella pneumoniae 5,26±0,34 5,46±0,27 0,45 =0,65
Staphylococcus aureus 4,30±0,13 2,89±0,41 2,57 =0,01

In a drawing 28 the qualitative and quantitative structure of an intestinal microflora according to feces crops on a dysbacteriosis depending on presence LN is shown.


WHICH/G

12,00 11,00

10,64


10,00

8,00

6,00

4,00

7,00

6,64

4,69
5,26 5,26

4,38 4,48

5,46

4,30

2,89

2,00

0,00

LN without LN

the Drawing 28. Qualitative and quantitative structure of an intestinal microflora depending on presence LN according to feces crops on a dysbacteriosis

the Analysis of data of crops of a feces on a dysbacteriosis depending on age has not taped authentic differences at children OG depending on age. However the quantity bifidobaktery, laktobaktery is taped, that, Klebsiella pneumoniać and Staphylococcus aureus was above at the age of 1-2 months, and Citrobacter freundii and Klebsiella oxytoca - in 3-4 months. Results of crops of a feces on a dysbacteriosis are presented in table 28.

Table 28. Results of crops of a feces on a dysbacteriosis depending on age

Microorganisms 1-2 months

(n=20)

3-4 months

(n=25)

5-6 months

(n=17)

t p

Bifidobakterii

11,00±0,19

10,64±0,17

10,64±0,25

t 1-3 =1,41 =0,16
t 1-5 =1,16 =0,25
t 3-5 =0,02 =0,98

Laktobakterii

6,90±0,10

6,72±0,13

6,70±0,20

t 1-3 =1,02 =0,31
t 1-5 =0,88 =0,38
t 3-5 =0,06 =0,95

Citrobacter freundii

4,55±0,30

4,64±0,26

4,23±0,23

t 1-3 =0,22 =0,82
t 1-5 =0,79 =0,43
t 3-5 =1,08 =0,28

Klebsiella oxytoca

4,60±0,32

5,00±0,35

4,64±0,35

t 1-3 =0,81 =0,41
t 1-5 =0,09 =0,92
t 3-5 =0,68 =0,49
Klebsiella pneumoniae

5,50±0,35

5,28±0,35

5,41±0,42

t 1-3 =0,43

t 1-5 =0,16

t 3-5 =0,23

=0,66

= 0,87

= 0.81

Staphylococcus aureus

4,15±0,35

3,08±0,46

3,05±0,59

t 1-3 =1,76

t 1-5 =1,64

t 3-5 =0.02

0,08

= 0,10

= 0,97

In a drawing 29 the qualitative and quantitative structure of an intestinal microflora depending on age according to feces crops on a dysbacteriosis is shown.


WHICH/G

12,00 11,00

10,00

8,00

6,00

4,00

2,00

0,00

10,64

10,64

6,90 6,72

6,70

4,55

4,64

4,23

4,60 5,00

4,64

5,50

5,28

5,41

4,15 3,08

3,05

1-2 mes 3-4 mes 5-6 mes

the Drawing 29. Qualitative and quantitative structure of an intestinal microflora depending on age according to feces crops on a dysbacteriosis

By results of feces crops on a dysbacteriosis depending on a feeding kind the quantity bifidobaktery is taped, that, laktobaktery and Staphylococcus aureus was authentically above at children who are on thoracal feeding. Quantity Citrobacter freundii was authentically above on artificial feeding. Quantity Klebsiella pneumoniać and Klebsiella oxytoca had no authentic differences depending on a feeding kind. Results of crops of a feces on a dysbacteriosis depending on a feeding kind are presented in table 29.

Table 29. Results of crops of a feces on a dysbacteriosis depending on a feeding kind

Microorganisms Thoracal

feeding (n=42)

Artificial

feeding (n=20)

t p
Bifidobakterii 10,90±0,09 10,45±0,22 2,20 =0,03
Laktobakterii 6,92±0,07 6,45±0,19 2,80 =0,007
Citrobacter freundii 4,28±0,14 4,95±0,35 2,02 =0,04
Klebsiella oxytoca 4,69±0,24 4,95±0,35 0,60 =0,54
Klebsiella pneumoniae 5,61±0,25 4,90±0,39 1,59 =0,11
Staphylococcus aureus 3,78±0,30 2,65±0,52 1,97 =0,05

In a drawing 30 the qualitative and quantitative structure of an intestinal microflora depending on a kind of feeding according to feces crops on a dysbacteriosis is shown.

WHICH/G


15,00

10,00

5,00

10,90

10,45

6,92

6,45

4,28

4,95

4,69

4,95

5,61

4,90

3,78

2,65

0,00

Thoracal feeding Artificial feeding

the Drawing 30. Qualitative and quantitative structure of an intestinal microflora depending on a kind of feeding according to feces crops on a dysbacteriosis

the Analysis of results of crops of a feces on a dysbacteriosis depending on presence MKK has shown, that the quantity bifidobaktery and laktobaktery was authentically above at children with MKK. Quantity Citrobacter freundii, Klebsiella oxytoca, Klebsiella pneumoniae and Staphylococcus aureus had no authentic differences. Results of crops of a feces on a dysbacteriosis depending on presence MKK are presented in table 30.

Table 30. Results of crops of a feces on a dysbacteriosis depending on presence MKK

Microorganisms MKK (n=44) Without MKK (n=18) t p
Bifidobakterii 10,95±0,04 10,27±0,30 3,33 =0,001
Laktobakterii 6,95±0,4 6,33±0,24 3,67 =0,001
Citrobacter freundii 4,68±0,21 4,05±0,05 1,84 =0,07
Klebsiella oxytoca 4,81±0,24 4,66±0,33 0,34 =0,73
Klebsiella pneumoniae 5,40±0,25 5,33±0,40 0,16 =0,87
Staphylococcus aureus 3,52±0.31 3,16±0,57 0,58 =0,56

In a drawing 31 the qualitative and quantitative structure of an intestinal microflora depending on presence MKK according to feces crops on a dysbacteriosis is shown.


WHICH/G

12,00 10,95

10,00

8,00

6,00

4,00

2,00

0,00

10,27

6,95

6,33

4,68 4,05 4,81

4,66

5,40

5,33

3,52

3,16

MKK without MKK

the Drawing 31. Qualitative and quantitative structure of an intestinal microflora depending on presence MKK according to feces crops on a dysbacteriosis

Thus, the quantity bifidobaktery at children OG, according to feces crops on a dysbacteriosis, was within norm, above at children with LN (11,00±0,23 WHICH/G) and at the age of 1-2 months (11,00±0,19 WHICH/G). The quantity laktobaktery in investigated groups was within norm, above at children with LN (7,00±0,09 WHICH/G). Quantity Citrobacter freundii was raised in all groups, above at children on artificial feeding (4,95±0,35 WHICH/G). Quantity Klebsiella oxytoca was raised in all groups, above at children with LN (5,26±0,40 WHICH/G). Quantity Klebsiella pneumoniać was raised in all groups, above at children on thoracal feeding (5,61±0,25 WHICH/G). Staphylococcus aureus it is found out in all groups, with the greatest quantity in children with LN (4,30±0,13 WHICH/G). In a drawing 32 the qualitative and quantitative structure of an intestinal microflora according to feces crops on a dysbacteriosis is shown.

without MKK

10,27

6,33

4,05

4,66

5,33 3,16

Bifidobakterii

MKK 10,95 6,95 4,68 4,81 5,40 3,52

isk.vsk.

10,45

6,45

4,95

4,95

4,90 2,65

Laktobakterii
grud.vsk 10,90 6,92 4,28 4,69 5,61 3,78

5-6 mes

10,64

6,70

4,23

4,64

5,41 3,05

Citrobacter freundii
3-4 mes 10,64 6,72 4,64 5,00 5,28 3,08
Klebsiella oxytoca
1-2 mes 11,00 6,90 4,55 4,60 5,50 4,15
without LN 10,61 6,64 4,38 4,48 5,46 2,89
Klebsiella
LN 11,00 7,00 4,69 5,26 5,26 4,30 pneumoniae
KG 10,20 6,55 4,00 4,70 4,82 2,15

Staphylococcus

OG 10,75 6,77 4,50 4,77 5,38 3,41 aureus
WHICH/G 0,00 5,00 10,00 15,00 20,00 25,00 30,00 35,00 40,00

the Drawing 32. Qualitative and quantitative structure of an intestinal microflora in investigated groups according to feces crops on a dysbacteriosis

Results PTSR of a feces

As a result of the analysis of data PTSR of a feces it is taped, that the quantity bifidobaktery and Klebsiella pneumoniae was authentic more low in OG, than in KG, and quantity Clostridium difficile, Enterococcus spp. And Faecalibacterium prausnitzii was above. The Total of microbes, quantity laktobaktery and Bacteroides fragilis had no authentic differences. Results PTSR of a feces in OG and KG are presented in table 31.

Table 31. Results PTSR of a feces in OG and KG

Microorganisms OG (n=62) KG (n=40) t p
Total of microbes 10,10±0,09 10,19±0,10 0,66 =0,50
Bifidobakterii 8,71±0,14 9,41±0,14 3,31 =0,001
Laktobakterii 6,14±0,29 6,21±0,20 0,17 =0,86
Bacteroides fragilis 8,48±0,19 8,39±0,14 0.33 =0,74
Clostridium difficile 4,78±0,28 3,32±0,13 3,93 < 0,001
Enterococcus spp. 5,54±0,08 4,67±0,21 4,32 < 0,001
Klebsiella pneumoniae 4,26±0,11 4,79±0,14 2,91 =0,005
Faecalibacterium prausnitzii 4,29±0,32 3,00±0,28 2,82 =0,006

In a drawing 33 the qualitative and quantitative structure of an intestinal microflora according to PTSR a feces in OG and KG is shown.

WHICH/G


12,00

10,00

8,00

6,00

4,00

2,00

0,00

10,10

10,19

8,71

9,41

6,14

6,21

8,48

8,39

4,78

3,32

5,54

4,29
4,67 4,79

4,26

3,00

OG KG

the Drawing 33. Qualitative and quantitative structure of an intestinal microflora according to PTSR feces OG and KG

By results of PTSR a feces it has been taped, that quantity Enterococcus spp. At children with FR without LN was authentically above, than with LN. Quantity Klebsiella pneumoniae was authentically above at LN. The Total of microbes, quantity bifidobaktery, laktobaktery, Bacteroides fragilis, Clostridium difficile and Faecalibacterium prausnitzii had no authentic differences. Results PTSR of a feces depending on presence LN are presented in table 32.

Table 32. Results PTSR of a feces depending on presence LN

Microorganisms FR with LN

(n=23)

FR without LN

(n=39)

t p
Total of microbes 10,05±0,16 10,13±0,10 0,43 =0,66
Bifidobakterii 8,51±0,25 8,83±0,16 1,08 =0,28
Laktobakterii 5,97±0,44 6,24±0,39 0,44 =0,65
Bacteroides fragilis 8,21±0,38 8,64±0,20 1,08 =0,28
Clostridium difficile 4,58±0,44 4,90±0,37 0,52 =0,60
Enterococcus spp. 5,21±0,08 5,73±0,10 3,32 =0,002
Klebsiella pneumoniae 4,66±0,27 4,03±0,03 2,88 =0,006
Faecalibacterium prausnitzii 4,31±0,54 4,28±0,41 0,05 =0,95

In a drawing 34 the qualitative and quantitative structure of an intestinal microflora according to PTSR depending on presence LN is shown.


WHICH/G

12,00

10,05

10,00
8,00
6,00
4,00
2,00
0,00

10,13

8,51 8,83

5,97

6,24

8,21

8,64

4,58

4,90

5,21 5,73 4,66

4,03

4,31

4,28

LN without LN

the Drawing 34. Qualitative and quantitative structure of an intestinal microflora according to PTSR a feces depending on presence LN

By results of PTSR a feces depending on age it has been taped, that quantity Clostridium difficile was authentically above at the age of 1-2 months. Quantity Enterococcus spp. Was authentically above in 5-6 months. Quantity Faecalibacterium prausnitzii was authentically above in 1-2 months. The total of microbes, quantity laktobaktery, Clostridium difficile, Klebsiella pneumoniać and Bacteroides fragilis had no authentic differences depending on age. Results PTSR of a feces depending on age are presented in table 33.

Table 33. Results PTSR of a feces depending on age

Mikro - organisms 1-2 months (n=20) 3-4 months (n=25) 5-6 months (n=17) t p
the General

10,13±0,14

9,00±0,15

10,22±0,16

t 1-3 =0,63 =0,52
quantity t 1-5 =0,41 =0,68
microbes t 3-5 =0,99 =0,32

Bifido-bacteria

8,71±0,28

8,76±0,17

8,65±0,30

t 1-3 =0,15 =0,88
t 1-5 =0,15 =0,88
t 3-5 =0,33 =0.74

Laktobakterii

6,06±0,45

5,99±0,43

6,45±0.70

t 1-3 =0,10 =0,92
t 1-5 =0,48 =0,62
t 3-5 =0,58 =0.56
Bacteroides fragilis

8,65±0,30

8,14±0,38

8,78±0,20

t 1-3 =1,00

t 1-5 =0,33

t 3-5 =1,30

=0,31

= 0.73

= 0,20

Clostridium difficile

3,93±0,38

5,23±0,50

5,12±0,51

t 1-3 =1,95

t 1-5 =1,88

t 3-5 =0,14

=0,05

= 0,06

= 0,88

Enterococcus spp.

5,20±0,09

5,60±0,10

5,85±0,20

t 1-3 =2,88 < 0,001
t 1-5 =3,08 < 0,001
t 3-5 =1,24 =0,21
Klebsiella pneumoniae

4,61±0,29

4,12±0,12

4,08±0,08

t 1-3 =1,67

t 1-5 =1,60

t 3-5 =0,20

=0,10

= 0,11

= 0,84

Faecali-bacterium prausnitzii

5,13±0,67

4,26±0,52

3,35±0,35

t 1-3 =1,04

t 1-5 =2,22

t 3-5 =1,29

=0,30

= 0,03

= 0,20


In a drawing 35 the qualitative and quantitative structure of an intestinal microflora depending on age according to PTSR a feces is shown.


WHICH/G

12,00 10,13

10,22

10,00

8,00

9,00 8,718,76

8,65

8,65 8,14 8,78

5,99 6,45

6,00 5,23 5,12 5,20 4,614,12

3,93 4

4,00
2,00
0,00

6,06

5,60 5,85

, 08

5,13

4,26

3,35

1-2 mes 3-4 mes 5-6 mes

the Drawing 35. Qualitative and quantitative structure of an intestinal microflora depending on age according to PTSR a feces

the Analysis of results PTSR of a feces depending on a feeding kind has shown, that quantity Clostridium difficile and Enterococcus spp was authentically above at children on artificial feeding. Quantity Faecalibacterium prausnitzii was authentically above at children on thoracal feeding. The total of microbes, quantity bifidobaktery, laktobaktery, Bacteroides fragilis and Klebsiella pneumoniae had no authentic differences. Results PTSR of a feces depending on a feeding kind are presented in table 34.

Table 34. Results PTSR of a feces depending on a feeding kind

Microorganisms Thoracal feeding (n=42) Artificial feeding (n=20) t p
Total

microbes

10,05±0,11 10,20±0,14 0,74 =0,45
Bifidobakterii 8,59±0,18 8,96±0,18 1,23 =0,22
Laktobakterii 5,85±0,32 6,74±0,59 1,41 =0,16
Bacteroides fragilis 8,70±0,21 8,02±0,38 1,63 =0,09
Clostridium difficile 4,07±0,27 6,26±0,54 4,02 < 0,001
Enterococcus spp. 5,26±0,06 6,13±0,13 6,48 < 0,001
Klebsiella pneumoniae 4,39±0,16 4,00±0,21 1,43 =0,16
Faecalibacterium

prausnitzii

4,91±0,45 3,00±0,31 4,68 < 0,001

In a drawing 36 the qualitative and quantitative structure of an intestinal microflora depending on a kind of feeding according to PTSR a feces is shown.

WHICH/G


12,00 10,05

10,00

8,00

6,00

4,00

2,00

0,00

10,20

8,59

8,96

5,85

6,74

8,70

8,02

4,07

6,26 6,13

5,26

4,39 4,00

4,91

3,00

Thoracal feeding Artificial feeding

the Drawing 36. Qualitative and quantitative structure of an intestinal microflora depending on a kind of feeding according to PTSR a feces

the Analysis of results PTSR of a feces depending on presence MKK has shown, that quantity Faecalibacterium prausnitzii was authentically above at children with MKK, and quantity Enterococcus spp. Was above at children without MKK. Total of microbes, bifidobaktery, Bacteroides fragilis, Clostridium difficile and Klebsiella pneumoniać had no authentic differences. Results PTSR of a feces depending on presence MKK are presented in table 35.

Table 35. Results PTSR of a feces depending on presence MKK

Microorganisms MKK

(n=44)

Without MKK

(n=18)

t p
Total of microbes 10,12±0,11 10,05±0,12 0,34 =0,73
Bifidobakterii 8,80±0,16 8,49±0,26 1,00 =0,31
Laktobakterii 6,07±0,32 6,31±0,64 0,36 =0,71
Bacteroides fragilis 8,49±0,23 8,45±0,32 0,09 =0,92
Clostridium difficile 4,46±0,32 5,56±0,52 1,79 =0,07
Enterococcus spp. 5,38±0,07 5,92±0,18 3,23 =0,002
Klebsiella pneumoniae 4,34±0,15 4,08±0,08 1,08 =0,28
Faecalibacterium prausnitzii 4,68±0,42 3,33±0,33 1,92 =0,05

In a drawing 37 the qualitative and quantitative structure of an intestinal microflora depending on presence MKK according to PTSR a feces is shown.


WHICH/G

12,00 10,12

10,00

8,00

10,05

8,80

8,49

6,07

6,31

8,49

8,45

5,56 5,92

6,00

4,00

2,00

0,00

4,46 5,38 4,34

4,08

4,68

3,33

MKK without MKK

the Drawing 37. Qualitative and quantitative structure of an intestinal microflora depending on presence MKK according to PTSR a feces

In a drawing 38 the qualitative and quantitative structure of an intestinal microflora in investigated groups according to PTSR a feces is shown.

without MKK 10,05 8,49 6,31 8,45 5,56 5,92 4,08 3,33 the General kol-in microbes
MKK 10,12 8,80 6,07 8,49 4,46 5,38 4,34 4,68 Bifidobakterii
iskus.vsk. 10,20 8,96 6,74 8,02 6,26 6,13 4,00 3,00
Laktobakterii
grud.vsk 10,05 8,59 5,85 8,70 4,07 5,26 4,39 4,91
5-6 mes 10,22 8,65 6,45 8,78 5,12 5,85 4,08 3,35 Bacteroides fragilis
3-4 mes 9,00 8,76 5,99 8,14 5,23 5,60 4,12 4,26
Clostridium difficile
1-2 mes 10,13 8,71 6,06 8,65 3,93 5,20 4,61 5,13
without LN 10,13 8,83 6,24 8,64 4,90 5,73 4,03 4,28 Enterococcus spp.
LN 10,05 8,51 5,97 8,21 4,58 5,21 4,66 4,31

Klebsiella

KG 10,19 9,41 6,21 8,39 3,32 4,67 4,79 3,00 pneumoniae
Faecalibacterium
OG 10,10 8,71 6,14 8,48 4,78 5,54 4,26 4,29 prausnitzii
WHICH/G 0,00 10 , 00 20,00 30,00 40,00 50,00 60,00

the Drawing 38. Qualitative and quantitative structure of an intestinal microflora in investigated groups according to PTSR a feces

Thus, the total of microbes was above at children at the age of 5-6 months (10,22±0,16 WHICH/G). The quantity bifidobaktery has been lowered in all groups, above in KG (9,41±0,14 WHICH/G). The quantity laktobaktery is lowered at children on thoracal feeding (5,85±0,32 WHICH/G), above at children on artificial feeding (6,74±0,59 WHICH/G). Quantity Bacteroides fragilis in all groups was within norm, above at children with FR at the age of 5-6 months (8,78±0,20 WHICH/G). Quantity Clostridium difficile was raised in all groups, above at children on artificial feeding (6,26±0,54 WHICH/G). Quantity Enterococcus spp. In all groups was within norm, above at children with FR on artificial feeding (6,13±0,13 WHICH/G). Quantity Klebsiella pneumoniać is raised in all groups, above at children with LN (4,66±0,27 WHICH/G). Quantity Faecalibacterium prausnitzii was above at children with FR at the age of 1-2 months (5,13±0,67 WHICH/G).

the Comparative estimation of results of crops of a feces on a dysbacteriosis and PTSR a feces

it is taped. Comparison of results of crops of a feces on a dysbacteriosis and PTSR real time at LN is presented in table 36. a feces in
Table 36. Comparison of results of crops of a feces on a dysbacteriosis and PTSR a feces at FR with LN (n=23)

We had been spent a comparative estimation of results of crops of a feces on a dysbacteriosis and PTSR a feces of identical species of microorganisms. As a result of comparison of given these methods at children with LN it is taped, that the quantity bifidobaktery and laktobaktery is authentic more low according to PTSR a feces in real time, than according to feces crops on a dysbacteriosis. Quantity Klebsiella pneumoniać also was more low according to PTSR a feces, but authentic differences not

Microorganisms

research Methods

td

p

feces crops on a dysbacteriosis

PTSR a feces

Bifidobakterii 11,00±0,23 8,51±0,25 9,76 < 0,001
Laktobakterii 7,00±0,09 5,97±0,44 2,28 =0,032
Klebsiella pneumoniae 5,26±0,34 4,66±0,27 1,22 =0,23

the Quantity bifidobaktery and Klebsiella pneumoniać is authentic more low according to PTSR a feces, than according to feces crops on a dysbacteriosis. The quantity laktobaktery also was more low according to PTSR a feces, but authentic differences is not taped. Comparison of results of crops of a feces on a dysbacteriosis and PTSR a feces at LN is presented in table 37.

Microorganisms

research Methods

td

p

feces crops on a dysbacteriosis PTSR a feces
Bifidobakterii 10,61±0,15 8,83±0,16 8,83 < 0,001
Laktobakterii 6,64±0,12 6,24±0,39 0,95 =0,34
Klebsiella pneumoniae 5,26±0,34 4,03±0,03 5,31 < 0,001

Table 37. Comparison of results of crops of a feces on a dysbacteriosis and PTSR a feces at FR without LN (n=39)

Thus, results PTSR and feces crops on a dysbacteriosis show different results. At FR GASTROINTESTINAL TRACT, irrespective of presence LN, quantity of microorganisms, according to PTSR a feces in real time, more low, than according to feces crops on a dysbacteriosis. It is especially important for an estimation of an obligate microflora which has appeared lowered at all children with FR GASTROINTESTINAL TRACT.

Results of definition of level kalprotektina in a feces

the Average level kalprotektina in OG has made 344,58±25,32 mkg/g, that authentically above, than in KG - 82,92±6,47 mkg/g (p< 0,001). In a drawing 39 level kalprotektina in OG and KG is shown.


350

300

250

200

150

100

50

0

344,58

OG KG

82,92

the Drawing 39. Level kalprotektina in OG and KG

Level kalprotektina at children with LN (522,21±27,84 the mkg/g) was authentically above, than without LN (239,82±24,35 mkg/g; p< 0,001). In a drawing 40 level kalprotektina depending on presence LN is shown.


600

500

522,21


400

300

200

239,82

100

0

LN without LN

the Drawing 40. Level kalprotektina depending on presence LN

Level kalprotektina at the age of 1-2 months was authentically above, than in 5-6 months. Level kalprotektina at the age of 3-4 months was authentically above, than in 5-6 months. Level kalprotektina depending on age is presented in table 38.

Table 38. Level kalprotektina depending on age

Level kalpro - tektina (mkg/g) 1-2 months (n=20) 3-4 months (n=25) 5-6 months (n=17)

t

p

448,10±42,43

373,36±33,00

180,47±38,07

t 1-3 =1,41

t 1-5 =4,62

t 3-5 =3,79

=0,16

AND lt; 0,001

AND lt; 0,001

In a drawing 41 level kalprotektina depending on age is shown.


500

450

400

350

300

250

200

150

100

50

0

448,1

373,36

180,47

1-2 mes 3-4 mes 5-6 mes

the Drawing 41. Level kalprotektina depending on age

Level kalprotektina at children on thoracal feeding has made 377,64±30,5 mkg/g, that authentically above, than at children on artificial feeding - 275,15±42,2 mkg/g (p=0,05). In a drawing 42 level kalprotektina depending on a feeding kind is shown.


400

350

300

250

200

150

100

50

0

377,64

275,15

grudn.vsk. iskusst.vsk.

the Drawing 42. Level kalprotektina depending on a feeding kind

Level kalprotektina at children with MKK has made 396,36±27,7 mkg/g, that authentically above, than without MKK - 218,00±42,98 mkg/g (p=0,001).


MKK.

In a drawing 43 level kalprotektina depending on presence

is shown


400,00

396,36


300,00

200,00

218,00

100,00


0,00

MKK without MKK

the Drawing 43. Level kalprotektina depending on presence MKK

Thus, level kalprotektina was raised in all investigated groups except KG. The highest level kalprotektina became perceptible at children with LN (522,21±27,8 mkg/g). We ranged level kalprotektina from maximum to normal depending on presence LN, age, a kind vskarmdivanija. Level kalprotektina in investigated groups is shown in a drawing 44.


mkg/g

600,00

500,00

400,00

300,00

200,00

100,00

522,21

448,10

396,36 377,64 373,36

344,58

272,15 239,82

218,00

180,47

82,92


0,00

LN 1-2ìćń MKK grud.vsk 3-4 mes OG isk.vsk without LN without MKK 5-6 mes KG

the Drawing 44. Level kalprotektina in investigated groups

Analyzing features of an intestinal microflora at children of early age with FRZHKT, it is possible to make following conclusions:

1. According to PTSR, at children of the first half of the year of a life with FRZHKT depression of representatives of an obligate intestinal microflora becomes perceptible: bifido - and laktobaktery.

2. At all children with FRZHKT as a part of an intestinal microflora the quantity of is conditional-pathogenic microbes is raised: Staphylococcus aureus, Enterococcus spp., Clostridium difficile, Citrobacter freundii, Klebsiella oxytoca and Klebsiella pneumoniać.

3. At children of the first half of the year of a life with FRZHKT the raised level kalprotektina in a feces (344,58±25,32 mkg/g) becomes perceptible, especially at LN, in first two months of a life, it above at children on thoracal feeding and at children with MKK.

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Scientific source KUBALOVA Saida Sultanovna. CLINICAL VALUE LAKTAZNOJ of INSUFFICIENCY And MICROBIC DISTURBANCES AT FUNCTIONAL DISORDERS of the GASTROENTERIC TRACT At CHILDREN of EARLY AGE. The dissertation on competition of a scientific degree of the candidate of medical sciences. St.-Petersburg - 2014. 2014

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Other medical related information Chapter 5 of FEATURE of the INTESTINAL MICROFLORA AT FUNCTIONAL DISORDERS of the GASTROENTERIC TRACT At CHILDREN of EARLY AGE:

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  2. Chapter 5 of FEATURE of the INTESTINAL MICROFLORA AT FUNCTIONAL DISORDERS of the GASTROENTERIC TRACT At CHILDREN of EARLY AGE
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  4. Chapter 5 of FEATURE of the INTESTINAL MICROFLORA AT FUNCTIONAL DISORDERS of the GASTROENTERIC TRACT At CHILDREN of EARLY AGE