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METHODS OF THE ASSESSMENT OF PHAGOCYTAL REACTION OF LEUCOCYTES

Estimated phagocytal reaction on a state nejtrofilnyh granulotsitov (NG). Testings-functions NG spent on following parametres:

- To quantity of leucocytes in peripheric blood;

- To absolute and relative number circulating NG in peripheric blood;

- To state retsep the even device on an expression of E-receptors NG to erythrocytes of the ram in test E-rozetkoobrazovanija (E-RON);

- Absorbing ability concerning latex particles;

- To state cytotoxic kislorodzavisimoj systems on indicators of the spontaneous NBT-test (the test with nitrodark blue tetrazoliem):

To average cytochemical quotient (СЦК/NBT).

The maintenance of leucocytes and lejkogrammu defined the standard

Method about use ’ the keyboard mechanical counter of leukocytic blood count SL 1 (Joint-Stock Company "ЛОиП", S.-Petersburg, 1999). The general number of leucocytes defined melanzhsrnym a mean with the subsequent inventory of cages in chamber Gorjaeva. To research of the leukocytic formula applied a mean of a coloration of Unguentums of peripheric blood a paint Romanovsky-gimza. Calculated relative and absolute quantity Ng.

State retseptornogo device NG and them adgezivnuju ability estimated on an expression neutrophils of E-receptors to erythrocytes of the ram in test E-rozegkoobrazovanija. Thus defined quantity E - rozetkoobrazujushchih neutrophils from 100 counted up (E ROI). Absorbing ability of YOKES estimated by relative and absolute quantity of awakely englobing cages - activity of an englobement (AF), i.e. abilities of neutrophils to absorb inert particles • melaminoformaldegidnogo latex in the dimension 1,5-2 microns. As a phagocyte considered the neutrophil which has absorbed of one and more particles of latex. Counted up quantity of phagocytes on 100 neutrophils and defined activity of an englobement in %. Cytotoxic kislorodzavisimuju activity of neutrophils defined them by training superoksidnogo

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Radical which revealed in the test of restoration nitrodark blue tetrazolija (NBT) — in spontaneous NBT - the test (Park, paravariation SHubich, 1981). The test is based on ability of practically pallid indicator NBT to be absorbed and be recovered to unsolvable dark blue dnformazaia in cytoplasm under the influence of awake forms of oxygen produced by phagocytes (AFK) which is easy for identifying in neutrophils visually. Thus intensity of a coloration is proportional to quantity of the formed Indicator reflecting cellular activity. Defined also average cytochemical quotient (СЦК/NBT).

Defined inte§raln§j an indicator - a load index (IN), testifying to intensity of immune system. This interrelation of indicators of activity of lymphocytes and neutrophils in tests E - rozetkoobrazovanija. It calculated under the formula: IN-E-ROL/E-RON.

2.5.2

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Scientific source RODIONOVA SVETLANA VIKTOROVNA. STUDYING of EFFICACYY of IMMUNOMODULATING FACTOR DERINATA In TREATMENT of CHRONIC JET ARTHRITISES HLAMIDIJNOJ of the ETIOLOGY At CHILDREN. The DISSERTATION on competition of a scientific degree of the candidate of medical sciences. Saransk 2007 . 2007

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